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Leptospira spp. : Methods and Protocols (Methods in Molecular Biology)

By: Mathieu Picardeau (Edited by) , Nobuo Koizumi (Edited by)

Extended Catalogue

Ksh 22,800.00

Format: Paperback or Softback

ISBN-10: 1071604619

ISBN-13: 9781071604618

Collection / Series: Methods in Molecular Biology

Collection Type: Publisher collection

Edition statement: 2020 ed.

Publisher: Springer-Verlag New York Inc.

Imprint: Springer-Verlag New York Inc.

Country of Manufacture: GB

Country of Publication: GB

Publication Date: Jul 7th, 2021

Publication Status: Active

Product extent: 291 Pages

Product Classification / Subject(s): Diseases & disorders
Microbiology (non-medical)

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This volume looks at all aspects of manipulation of Leptospira spp. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from both clinical and environmental samples; using methods like whole genome sequencing and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry to identify bacterial species; tools for gene inactivation and in vitro and in vivo assays to study the pathogenesis of leptospirosis; and the use of hamsters to evaluate leptospiral virulence and vaccine candidates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Leptospira spp.: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms.  

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